Part of RNA processing in eukaryotes is addition of the 5′ mRNA Cap, which is essential for mRNA stability, transport, and translation (by guiding ribosomal assembly at the RNA’s 5′ end). To ensure that only RNAP II transcripts are capped, the RNAP II CTD is phosphorylated, and the phosphorylated CTD recruits capping enzymes. After ~25-30 [...]

Eukaryotes encode long precursor RNA transcripts, which are spliced, polyadenylated and capped to produce a messenger RNA that can be translated. Capping Nucleus Guanosine is added to the 5′ end of the mRNA for stability, transport and translation initiation. Polyadenylation Nucleus The 3’ end of the RNA is cleaved and a Poly-A tail is added [...]