What Is A Hydrogenosome? Hydrogenosomes metabolize carbohydrates into ATP and H2. Metabolism in the hydrogenosome is similar to anaerobic bacteria (via enzymes including PFO and ferredoxin) and mitochondria (via Krebs cycle enzymes that convert acetyl CoA into acetate and succinyl CoA which is then converted to succinate and ATP). A Common Ancestor? Mitochondria and hydrogenosomes [...]

Isolation To isolate an aerobic N2 fixer, plate soil with a nitrogen sources (ammonia), O2 and carbon reducer. Incubate. Plate on NH3 agar, -NH3 agar, and MA. Our desired bacteria will grow on all but -NH3 agar. No eukaryotes aerobically fix N2. Azotobacter is an example of a nitrogen fixer. Ethylene is a nitrogenase substrate.

Methanogens are obligate anaerobic archaea that either consume or produce CO2, producing CH4. Methanotrophs consume CH4. Requirements for growth: Anoxic sediments Animal digestive systems (rumen of cattle, sheep, elk, camels) Geothermal sources of H2 + CO2 Artificial biodegradation facilities Endozymbiots of anaerobic protozoa As herbivores, they live off the complex carbohydrate cellulose. Methane produced in [...]