Motility



Cell Recognition

When two cells bind, it can happen 2 ways: Homotypic The same molecule sticks out of both cells. Heterotypic Two different molecules stick out of the cells.

Flagella Experiment

To determine which flagellar component was most important, the following experiment was performed. Cells are transposon tagged. Cells are incubated with an attractant at the top of the flask. Cells at the bottom are isolated and incubated in a flask with an attractant at the top. Cells at the bottom are isolated and incubated in [...]

Motility

Cells move to find food, shelter and safety. Flagellated Flagella are polar, bipolar, lophotrichous (bunch at 1 end), monotrichous, amphitricious and petrichous (E. coli). To view with high contrast, use die, electric microscope, or dark field. Help w/ attachment. cilia lophotrophs polar bilpolar Random Walk Cells move toward attractants (c-source), from repellent (organo acid) and [...]

Eukaryotic Flagella & Cilia

Length # Per Cell Motion Flagella Long; > 5µm 1-2/cell Whiplike Cilia Short; <5µm 100s/cell Breast stroke Axoneme (9+2 structure, MT bundle) is 9 pairs of microtubules around central pair. Microtubules are made of a- and B-tubulin). Surrounded by cell membrane. Dynein, ATP-dependent molecular motor, attached to tubulin. Whip-like motion. Cilia are structurally the same. [...]