Oxidase Test


Written by      First published October 18, 2008      Last modified October 18, 2008

The oxidase test assays for the presence of cytochrome c in a cell’s respiratory pathway. Cells are smeared onto a sheet coated with tetrametyl-p-phenylenediamine (TMP), a colorless electron donor. A functional cytochrome c removes electrons from TMP; this oxidized TMP has an intense purple-blue color. A positive control for the oxidase test is Pseudomonas fluorescens, and a negative control for the oxidase test is S. epidermidis.




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