Reporter GeneComments
parentGenetic techniques
siblings5'-Deletion MutantsAmes TestcDNA MicroarrayCloning VectorsConjugationDNA FingerprintingDNA MiniprepGel Shift AssayGene Control in Development: Laboratory TechniquesGene TargetingGenetic EngineeringGenetic screenIn Vitro Nuclear Run-on ExperimentInterrupted Mating ExperimentKnockout mutationLinkage analysisPolymerase Chain ReactionPromoter (Transcriptional) (RNA) FusionRestriction Enzymes (Endonucleases )Sequence AlignmentShotgun sequencingTemperature Sensitive Mutant ExperimentTransformationTransgenesTranslational (Protein) FusionTransposon Tagging

Reporter Gene

Reporter genes are fused to DNA fragments from a test gene or promoter. Reporter genes must be easily measured enzymatically or visually. Two common reporter genes are Β-galactosidase, green fluorescent protein (GFP) and yellow fluorescent protein (YFP). Β-gal is measured enzymatically using either X-gal (which Β-gal cleaves into a blue compound) or ONPG (which Β-gal cleaves into a yellow compound). GFP and YFP can be measured enzymatically, but are more easily measured visually because they fluoresce brightly.