Uridine Deletion/Insertion RNA Editing
By Levi Clancy for Student Reader on
updated
Cryptogene | A gene whose transcript is edited. |
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Guide RNA | (aka gRNA) A short 3'-uridylated RNA that can form a perfect duplex (except for the oligo[u] tail) with a stretch of mature edited mRNA. G-U base pairing is allowed. |
Anchor Duplex | The RNA duplex formed by hybridization of the 5' end of the gRNA and the mRNA sequence just downstream of the first editing site in an editing block. |
Pre-Edited | A pre-edited region or sequence will be edited in the mature mRNA. |
Unedited | An unedited region or sequence will never be edited in the mature mRNA. |
Editing Block | Edited mRNA sequence mediated by a single gRNA. |
Editing Domain | Edited mRNA sequence mediated by two or more overlapping gRNAs. |
Pan-Edited Gene | Extensively edited. |
3' oligo[U] Tail | The string of non-encoded U's at the 3' end of the gRNA |
gRNA-mRNA | A gRNA-mRNA chimera consists of a gRNA 5' end covalently bound to an mRNA 3' end, oft at an editing site. |
Model for U-Insertion RNA Editing
The gRNA's A's and U's guide addition of U's to the mRNA.
Hybrid | Hybridization of gRNA to mRNA just downstream of ES1. Perhaps mediated by an RNA chaperone. |
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Cut | Endonuclease cuts mRNA at mismatch. |
U Insertion | 3' TUTase (terminal uridyl transferase) adds U's to 3' end of 5' mRNA fragment. |
RNA Ligase | RNA ligase ligates the cut mRNA fragments back together. |
Model for U-Deletion RNA Editing
If the mRNA's U's are not base-paired in the gRNA, they are removed.
Hybrid | Hybridization of gRNA to mRNA just downstream of ES1. Perhaps mediated by an RNA chaperone. |
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Cut | Endonuclease cuts mRNA at mismatch. |
U Deletion | 3'-5' exonuclease removes bulged (unpaired) U's from mRNA. |
Ligation | RNA ligase ligates the cut mRNA fragments back together. |