Student Reader

Uridine Deletion/Insertion RNA Editing

CryptogeneA gene whose transcript is edited.
Guide RNA(aka gRNA) A short 3'-uridylated RNA that can form a perfect duplex (except for the oligo[u] tail) with a stretch of mature edited mRNA. G-U base pairing is allowed.
Anchor DuplexThe RNA duplex formed by hybridization of the 5' end of the gRNA and the mRNA sequence just downstream of the first editing site in an editing block.
Pre-EditedA pre-edited region or sequence will be edited in the mature mRNA.
UneditedAn unedited region or sequence will never be edited in the mature mRNA.
Editing BlockEdited mRNA sequence mediated by a single gRNA.
Editing DomainEdited mRNA sequence mediated by two or more overlapping gRNAs.
Pan-Edited GeneExtensively edited.
3' oligo[U] TailThe string of non-encoded U's at the 3' end of the gRNA
gRNA-mRNAA gRNA-mRNA chimera consists of a gRNA 5' end covalently bound to an mRNA 3' end, oft at an editing site.
Model for U-Insertion RNA Editing

The gRNA's A's and U's guide addition of U's to the mRNA.

HybridHybridization of gRNA to mRNA just downstream of ES1. Perhaps mediated by an RNA chaperone.
CutEndonuclease cuts mRNA at mismatch.
U Insertion3' TUTase (terminal uridyl transferase) adds U's to 3' end of 5' mRNA fragment.
RNA LigaseRNA ligase ligates the cut mRNA fragments back together.
Model for U-Deletion RNA Editing

If the mRNA's U's are not base-paired in the gRNA, they are removed.

HybridHybridization of gRNA to mRNA just downstream of ES1. Perhaps mediated by an RNA chaperone.
CutEndonuclease cuts mRNA at mismatch.
U Deletion3'-5' exonuclease removes bulged (unpaired) U's from mRNA.
LigationRNA ligase ligates the cut mRNA fragments back together.
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