(aka gRNA) A short 3'-uridylated RNA that can form a perfect duplex (except for the oligo[u] tail) with a stretch of mature edited mRNA. G-U base pairing is allowed.
Anchor Duplex
The RNA duplex formed by hybridization of the 5' end of the gRNA and the mRNA sequence just downstream of the first editing site in an editing block.
Pre-Edited
A pre-edited region or sequence will be edited in the mature mRNA.
Unedited
An unedited region or sequence will never be edited in the mature mRNA.
Editing Block
Edited mRNA sequence mediated by a single gRNA.
Editing Domain
Edited mRNA sequence mediated by two or more overlapping gRNAs.
Pan-Edited Gene
Extensively edited.
3' oligo[U] Tail
The string of non-encoded U's at the 3' end of the gRNA
gRNA-mRNA
A gRNA-mRNA chimera consists of a gRNA 5' end covalently bound to an mRNA 3' end, oft at an editing site.
Model for U-Insertion RNA Editing
The gRNA's A's and U's guide addition of U's to the mRNA.
Hybrid
Hybridization of gRNA to mRNA just downstream of ES1. Perhaps mediated by an RNA chaperone.
Cut
Endonuclease cuts mRNA at mismatch.
U Insertion
3' TUTase (terminal uridyl transferase) adds U's to 3' end of 5' mRNA fragment.
RNA Ligase
RNA ligase ligates the cut mRNA fragments back together.
Model for U-Deletion RNA Editing
If the mRNA's U's are not base-paired in the gRNA, they are removed.
Hybrid
Hybridization of gRNA to mRNA just downstream of ES1. Perhaps mediated by an RNA chaperone.
Cut
Endonuclease cuts mRNA at mismatch.
U Deletion
3'-5' exonuclease removes bulged (unpaired) U's from mRNA.
Ligation
RNA ligase ligates the cut mRNA fragments back together.