Polymerase Chain ReactionComments
parentGenetic techniques
siblings5'-Deletion MutantsAmes TestcDNA MicroarrayCloning VectorsConjugationDNA FingerprintingDNA MiniprepGel Shift AssayGene Control in Development: Laboratory TechniquesGene TargetingGenetic EngineeringGenetic screenIn Vitro Nuclear Run-on ExperimentInterrupted Mating ExperimentKnockout mutationLinkage analysisPromoter (Transcriptional) (RNA) FusionReporter GeneRestriction Enzymes (Endonucleases )Sequence AlignmentShotgun sequencingTemperature Sensitive Mutant ExperimentTransformationTransgenesTranslational (Protein) FusionTransposon Tagging

Polymerase Chain Reaction

The polymerase chain reaction (PCR) replicates specific genetic sequences (DNA or RNA, in either plus- or minus-sense) so that vast quantities of a certain DNA segment can be quickly produced (amplification). PCR relies upon a few basic steps:

  1. Obtain genetic material for PCR.
  2. Add oligonucleotide primers complementary to the sequence you wish to amplify.
  3. Single dNTPs are added to provide base pairs for newly synthesized DNA or RNA.
  4. Heat-stable polymerases are added to conduct the replication.
  5. The reaction is repeatedly heated and cooled, splitting and annealing DNA for the replication assembly to bind an conduct the replication.