By Levi Clancy for Student Reader on
- Genetic techniques
- 5'-Deletion Mutants
- Ames Test
- Cloning Vectors
- DNA Fingerprinting
- DNA Miniprep
- Gel Shift Assay
- Gene Control in Development: Laboratory Techniques
- Gene Targeting
- Genetic Engineering
- Genetic screen
- In Vitro Nuclear Run-on Experiment
- Interrupted Mating Experiment
- Knockout mutation
- Linkage analysis
- Polymerase Chain Reaction
- Promoter (Transcriptional) (RNA) Fusion
- Reporter Gene
- Restriction Enzymes (Endonucleases )
- Sequence Alignment
- Shotgun sequencing
- Temperature Sensitive Mutant Experiment
- Translational (Protein) Fusion
- Transposon Tagging
- cDNA Microarray
The polymerase chain reaction (PCR) replicates specific genetic sequences (DNA or RNA, in either plus- or minus-sense) so that vast quantities of a certain DNA segment can be quickly produced (amplification). PCR relies upon a few basic steps:
Obtain genetic material for PCR.
Add oligonucleotide primers complementary to the sequence you wish to amplify.
Single dNTPs are added to provide base pairs for newly synthesized DNA or RNA.
Heat-stable polymerases are added to conduct the replication.
The reaction is repeatedly heated and cooled, splitting and annealing DNA for the replication assembly to bind an conduct the replication.