Shotgun sequencing
By Levi Clancy for Student Reader on
updated
- Genetic techniques
- 5'-Deletion Mutants
- Ames Test
- Cloning Vectors
- Conjugation
- DNA Fingerprinting
- DNA Miniprep
- Gel Shift Assay
- Gene Control in Development: Laboratory Techniques
- Gene Targeting
- Genetic Engineering
- Genetic screen
- In Vitro Nuclear Run-on Experiment
- Interrupted Mating Experiment
- Knockout mutation
- Linkage analysis
- Polymerase Chain Reaction
- Promoter (Transcriptional) (RNA) Fusion
- Reporter Gene
- Restriction Enzymes (Endonucleases )
- Sequence Alignment
- Shotgun sequencing
- Temperature Sensitive Mutant Experiment
- Transformation
- Transgenes
- Translational (Protein) Fusion
- Transposon Tagging
- cDNA Microarray
Shotgun sequencing is a very fast process for finding the nucleotide sequence of a genome. The downside is that repeat sequences are poorly interpreted.
| Step | Overview |
|---|---|
| Isolate | Isolate chromosome from a homogeneous population. |
| Digest | Digest into 150kb sequence fragments. |
| Clone | Clone fragments into bacterial artificial chromosomes (BACs). |
| Transform | Transform BACs into recipients. |
| Screen | Screen for transformed colonies (selective media, like Xgal or CAM). |
| Sequence | Sequence the inserts. |
| Assemble | Assemble genome based on overlapping sequences. |
| Annotate | Genome annotation using BLAST (Basic Local Alignment Search Tool) |