Shotgun sequencingComments
parentGenetic techniques
siblings5'-Deletion MutantsAmes TestcDNA MicroarrayCloning VectorsConjugationDNA FingerprintingDNA MiniprepGel Shift AssayGene Control in Development: Laboratory TechniquesGene TargetingGenetic EngineeringGenetic screenIn Vitro Nuclear Run-on ExperimentInterrupted Mating ExperimentKnockout mutationLinkage analysisPolymerase Chain ReactionPromoter (Transcriptional) (RNA) FusionReporter GeneRestriction Enzymes (Endonucleases )Sequence AlignmentTemperature Sensitive Mutant ExperimentTransformationTransgenesTranslational (Protein) FusionTransposon Tagging

Shotgun sequencing

Shotgun sequencing is a very fast process for finding the nucleotide sequence of a genome. The downside is that repeat sequences are poorly interpreted.
IsolateIsolate chromosome from a homogeneous population.
DigestDigest into 150kb sequence fragments.
CloneClone fragments into bacterial artificial chromosomes (BACs).
TransformTransform BACs into recipients.
ScreenScreen for transformed colonies (selective media, like Xgal or CAM).
SequenceSequence the inserts.
AssembleAssemble genome based on overlapping sequences.
AnnotateGenome annotation using BLAST (Basic Local Alignment Search Tool)