Shotgun sequencing
Shotgun sequencing is a very fast process for finding the nucleotide sequence of a genome. The downside is that repeat sequences are poorly interpreted.
| Step | Overview |
|---|---|
| Isolate | Isolate chromosome from a homogeneous population. |
| Digest | Digest into 150kb sequence fragments. |
| Clone | Clone fragments into bacterial artificial chromosomes (BACs). |
| Transform | Transform BACs into recipients. |
| Screen | Screen for transformed colonies (selective media, like Xgal or CAM). |
| Sequence | Sequence the inserts. |
| Assemble | Assemble genome based on overlapping sequences. |
| Annotate | Genome annotation using BLAST (Basic Local Alignment Search Tool) |