DNA Fingerprinting
By Levi Clancy for Student Reader on
updated
- Genetic techniques
- 5'-Deletion Mutants
- Ames Test
- Cloning Vectors
- Conjugation
- DNA Fingerprinting
- DNA Miniprep
- Gel Shift Assay
- Gene Control in Development: Laboratory Techniques
- Gene Targeting
- Genetic Engineering
- Genetic screen
- In Vitro Nuclear Run-on Experiment
- Interrupted Mating Experiment
- Knockout mutation
- Linkage analysis
- Polymerase Chain Reaction
- Promoter (Transcriptional) (RNA) Fusion
- Reporter Gene
- Restriction Enzymes (Endonucleases )
- Sequence Alignment
- Shotgun sequencing
- Temperature Sensitive Mutant Experiment
- Transformation
- Transgenes
- Translational (Protein) Fusion
- Transposon Tagging
- cDNA Microarray
DNA fingerprinting (aka DNA profiling) is useful for: paternity testing; establishing twin zygosity; determining bone marrow transplant engraftment; identifying mislabeled pathology species (discomfortingly frequent); pedigree analysis of animals and animal products; and establishing identity of criminals. CODIX is an FBI-administered DNA index that catalogs the DNA fingerprints of individuals. DNA profiling can be done by PCR using primers that bind to the single copy sequences flanking particular minisatellite repeats. Amplification of polymorphic minisatellite sequences can identify tissue derived from specific individuals. Amplification of these sequences will show distinct patterns of products.