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Knockout mutationComments
parentGenetic techniques
siblings5'-Deletion MutantsAmes TestcDNA MicroarrayCloning VectorsConjugationDNA FingerprintingDNA MiniprepGel Shift AssayGene Control in Development: Laboratory TechniquesGene TargetingGenetic EngineeringGenetic screenIn Vitro Nuclear Run-on ExperimentInterrupted Mating ExperimentLinkage analysisPolymerase Chain ReactionPromoter (Transcriptional) (RNA) FusionReporter GeneRestriction Enzymes (Endonucleases )Sequence AlignmentShotgun sequencingTemperature Sensitive Mutant ExperimentTransformationTransgenesTranslational (Protein) FusionTransposon Tagging

Knockout mutation

One can use recombinant DNA techniques to make a "knockout" mutation in the gene in an embryonic stem (ES) cell line (one selects for replacement, by homologous recombination, of a wild type copy of the gene by an engineered mutant form of the gene). This mutated cell can then be introduced into a blastocyst; from this, chimeric mice, and after appropriate crosses, germ cells carrying the mutated gene can be obtained.