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5'-Deletion MutantsComments
parentGenetic techniques
siblingsAmes TestcDNA MicroarrayCloning VectorsConjugationDNA FingerprintingDNA MiniprepGel Shift AssayGene Control in Development: Laboratory TechniquesGene TargetingGenetic EngineeringGenetic screenIn Vitro Nuclear Run-on ExperimentInterrupted Mating ExperimentKnockout mutationLinkage analysisPolymerase Chain ReactionPromoter (Transcriptional) (RNA) FusionReporter GeneRestriction Enzymes (Endonucleases )Sequence AlignmentShotgun sequencingTemperature Sensitive Mutant ExperimentTransformationTransgenesTranslational (Protein) FusionTransposon Tagging

5'-Deletion Mutants

These mutations help determine the transcriptional control region of a gene. The 5' region keeps getting clipped away until transcription control is disrupted, thus determining the region.