Gel Shift AssayComments
parentGenetic techniques
siblings5'-Deletion MutantsAmes TestcDNA MicroarrayCloning VectorsConjugationDNA FingerprintingDNA MiniprepGene Control in Development: Laboratory TechniquesGene TargetingGenetic EngineeringGenetic screenIn Vitro Nuclear Run-on ExperimentInterrupted Mating ExperimentKnockout mutationLinkage analysisPolymerase Chain ReactionPromoter (Transcriptional) (RNA) FusionReporter GeneRestriction Enzymes (Endonucleases )Sequence AlignmentShotgun sequencingTemperature Sensitive Mutant ExperimentTransformationTransgenesTranslational (Protein) FusionTransposon Tagging

Gel Shift Assay

If the protein can recognize the sequence on an RNA or DNA, then the protein will shift up. Two different protein extracts bound to sequence & shifted it up on the gel. If a protein is bound to the sequence, it will shift up because it is heavier. Two different protein extracts & depending on which one you used from amastin gene based on 5' UTR or 3' UTR you found proteins bounds to the sequence & shifted it up on the gel.