Gel Shift Assay

If the protein can recognize the sequence on an RNA or DNA, then the protein will shift up. Two different protein extracts bound to sequence & shifted it up on the gel. If a protein is bound to the sequence, it will shift up because it is heavier. Two different protein extracts & depending on which one you used from amastin gene based on 5' UTR or 3' UTR you found proteins bounds to the sequence & shifted it up on the gel.