Gel Shift Assay
By Levi Clancy for Student Reader on
updated
- Parent
- Siblings
- 5'-Deletion Mutants
- Ames Test
- cDNA Microarray
- Cloning Vectors
- Conjugation
- DNA Fingerprinting
- DNA Miniprep
- Gene Control in Development: Laboratory Techniques
- Gene Targeting
- Genetic Engineering
- Genetic screen
- In Vitro Nuclear Run-on Experiment
- Interrupted Mating Experiment
- Knockout mutation
- Linkage analysis
- Polymerase Chain Reaction
- Promoter (Transcriptional) (RNA) Fusion
- Reporter Gene
- Restriction Enzymes (Endonucleases )
- Sequence Alignment
- Shotgun sequencing
- Temperature Sensitive Mutant Experiment
- Transformation
- Transgenes
- Translational (Protein) Fusion
- Transposon Tagging
If the protein can recognize the sequence on an RNA or DNA, then the protein will shift up. Two different protein extracts bound to sequence & shifted it up on the gel. If a protein is bound to the sequence, it will shift up because it is heavier. Two different protein extracts & depending on which one you used from amastin gene based on 5' UTR or 3' UTR you found proteins bounds to the sequence & shifted it up on the gel.