There are ~100 different known guide RNAs (aka gRNAs).
There is one guide RNA per minicircle species, and ~10,000 minicircles per kDNA network. There are multiple copies of each minicircle species. There are also maxicircle guide RNAs.
|Anchor Region||Hybridizes to the mRNA. Guide RNA hybridizes to mRNA at the anchor.|
|Guiding Region||Directs the U insertion and deletion. Guiding region contains mismatches.|
|Unpaired mRNA U's||These U's are deleted. There are no corresponding G's nor A's in the gRNA.|
|Unpaired gRNA G's & U's||A's are inserted into the mRNA to correspond to the G'a and U's in the gRNA.|
|Oligo U Tail||Stabilizes the 3' end of the gRNA. The oligo U tail varies in length. It is not the source of U's for insertion.|