Genetic Engineering
By Levi Clancy for Student Reader on
updated
- Genetic techniques
- 5'-Deletion Mutants
- Ames Test
- Cloning Vectors
- Conjugation
- DNA Fingerprinting
- DNA Miniprep
- Gel Shift Assay
- Gene Control in Development: Laboratory Techniques
- Gene Targeting
- Genetic Engineering
- Genetic screen
- In Vitro Nuclear Run-on Experiment
- Interrupted Mating Experiment
- Knockout mutation
- Linkage analysis
- Polymerase Chain Reaction
- Promoter (Transcriptional) (RNA) Fusion
- Reporter Gene
- Restriction Enzymes (Endonucleases )
- Sequence Alignment
- Shotgun sequencing
- Temperature Sensitive Mutant Experiment
- Transformation
- Transgenes
- Translational (Protein) Fusion
- Transposon Tagging
- cDNA Microarray
| Transgene | Cloned genes being introduced into host (usually eukaryotic). |
|---|---|
| Transgenic Organism | Organism receiving transgene. |
| Ectopic insertions | When transgenes are inserted randomly into genome. |
| Targeted Insertion | Targeted insertion (aka gene targeting) is when an inserted transgene replaces homologous sequence on genome. |
| Retroviruses | Advantages: integrates genes into host chromosomes, offering chance for long-term stability. Drawbacks: genes integrate randomly so might disrupt host genes; may infect only dividing cells. |
| Adenoviruses | Advantages: Most do not cause serious disease; large capacity for foreign genes. Drawbacks: genes may function transiently, owing to lack of integration or to attack by the immune system. |
| Adeno-associated viruses | Advantages: Integrate genes into host chromosomes; cause no known human diseases. Drawback: Small capacity for foreign genes. |