Interrupted Mating ExperimentComments

Interrupted Mating Experiment

The Interrupted Mating Experiment basically uses the following steps:

  1. Cells are mixed, then shaken to break their pili and stop conjugation.
  2. Streptomycin is added to stop cell division.
  3. Cells gain certain traits depending on which genes have been transferred.
  4. By stopping conjugation at different times, gene order of a plasmid and its inserts can be determined.